External Seminar Franck PEREZ

To regulate their homeostasis, cells need to address specific proteins and lipids toward particular compartments in a tightly controlled manner. Golgi-dependent trafficking plays a crucial role in these processes. Although our understanding of the mechanisms of intra-Golgi transport and sorting has significantly improved over the past two decades, the underlying complexity of transport routes, encompassing mechanisms and dynamics, is only beginning to be revealed. Over the years, we have developed several versatile assays to monitor and quantify the transport of cargoes to the cell surface, such as the RUSH assay and the CATCHFIRE system. These assays provide powerful tools for studying and perturbing cell dynamics in real-time. Recently, we applied these tools to gain a deeper understanding of how the cell surface PD-L1 is translocated through the secretory pathway and subsequently transported to the cell surface. Additionally, we leveraged our trafficking assays to develop novel translational approaches, screen for inhibitory molecules, and establish tunable cell-based therapies.